Consensus taxonomy: Staphylococcus epidermidis → Staphylococcus → Bacillales → Bacilli → Firmicutes → Bacteria
This genome is essentialy complete except for ~7.7kbp that are missing from the 3' side of 650. It has 5 16S rRNA encoding genes. This strain is infected by the 3 Staph epi phages (013, 014 and 4-6).
| contig | # features | sequence size | GC content (%) | Cov | Coding Density (%) | |
|---|---|---|---|---|---|---|
|
CARSEP_1
CARSEP_650
Species:
Staphylococcus epidermidis (72.96%)
|
651 | 739441 bp | 31.73 | 1.00 | 87.16 |
5': CARSEP_672 and then 23S:3. This is based on comparison to RP62A and the St. 3 genome. CARSEP_672 is equivalent to Scaf_530.
3': CARSEP_671:3' (equivalent to Scaf_550:(1-7771)
Itai Sharon
(11/18/12)
|
|
CARSEP_6
CARSEP_660
Species:
Staphylococcus epidermidis (77.68%)
|
475 | 554437 bp | 32.41 | 1.00 | 82.95 |
5': CARSEP_668:5'
3': CARSEP_668:5'
(497790, 498025) should be removed.
Itai Sharon
(11/18/12)
|
|
CARSEP_8
CARSEP_652
Species:
Staphylococcus epidermidis (71.43%)
|
126 | 126743 bp | 31.21 | 1.00 | 81.95 |
5': CARSEP_668:5'
3': CARSEP_647:3', integration site for Staph_epidermidis_phage_013 (5' side)
Itai Sharon
(11/18/12)
|
|
CARSEP_4
CARSEP_644
Species:
Staphylococcus epidermidis (81.08%)
|
111 | 121744 bp | 31.82 | 1.00 | 81.76 |
5': CARSEP_668:3
3': CARSEP_668:3
Itai Sharon
(11/18/12)
|
|
CARSEP_7
CARSEP_604
Species:
Staphylococcus epidermidis (73.33%)
|
90 | 97514 bp | 31.35 | 1.00 | 77.89 |
5': CARSEP_668:3
3': CARSEP_668:3
Itai Sharon
(11/18/12)
|
|
CARSEP_12
CARSEP_653
Species:
Staphylococcus epidermidis (74.12%)
|
85 | 83310 bp | 32.27 | 1.00 | 83.10 |
5': 16S:5
3': 23S:3
Itai Sharon
(11/18/12)
|
|
CARSEP_3
CARSEP_606
Species:
Staphylococcus epidermidis (64.63%)
|
82 | 71143 bp | 32.32 | 1.00 | 86.86 |
5': 16S:5
3': CARSEP_668:5
Itai Sharon
(11/18/12)
|
|
CARSEP_10
CARSEP_655
Species:
Staphylococcus epidermidis (83.54%)
|
79 | 84523 bp | 32.78 | 1.00 | 76.48 |
5': CARSEP_614:5'
3': 16S:5
Connection on 5' end is based on mini-assembly as well as alignment to RP62A with the later indicating that there's no missing piece between 614 to 655. Alignment to ATCC12228 indicates a missing piece of ~8Kb (ATCC12228(2464034, 2472461))
Itai Sharon
(11/18/12)
|
|
CARSEP_9
CARSEP_647
Species:
Staphylococcus epidermidis (85.71%)
|
77 | 83119 bp | 33.02 | 1.00 | 82.97 |
5': CARSEP_668:5'
3': CARSEP_652:3', integration site for Staph_epidermidis_phage_013 (3' end)
Itai Sharon
(11/18/12)
|
|
CARSEP_18
CARSEP_607
Species:
Staphylococcus epidermidis (73.61%)
|
72 | 70727 bp | 32.29 | 1.00 | 83.65 |
5': CARSEP_668:3
3': CARSEP_668:5
Itai Sharon
(11/18/12)
|
|
CARSEP_14
CARSEP_661
Species:
Staphylococcus epidermidis (79.37%)
|
63 | 64017 bp | 31.29 | 1.00 | 85.41 |
5': ~1.8 Kbp (possibly 666)->662:5
3': 16S:5
5' connection was established based on alignment to RP62A, in which 661:5 and 662:5 are 1.8 Kbp from each other and 666 may be between them (500bp are presnt in RP62A). Assembly probably did not work well enough at this point because the region at 661:5 is similar to 645 (a plasmid) at position ~40000.
Itai Sharon
(11/18/12)
|
|
CARSEP_17
CARSEP_613
Species:
Staphylococcus epidermidis (86.79%)
|
53 | 48143 bp | 31.27 | 1.00 | 81.87 |
5': CARSEP_668:5
3': CARSEP_668:3
Itai Sharon
(11/18/12)
|
|
CARSEP_20
CARSEP_614
Species:
Staphylococcus epidermidis (66.67%)
|
51 | 59240 bp | 32.81 | 1.00 | 79.03 |
5': CARSEP_655:5'
3': Scaf_654:5'
5' is based on alignment to RP62A in which 614:55781:3 is ~2Kbp away from 654:5. 614 has ~4 Kbp after 55781 which probably fill the gap.
Itai Sharon
(11/18/12)
|
|
CARSEP_2
CARSEP_646
Species:
Staphylococcus epidermidis (82.5%)
|
40 | 40512 bp | 33.38 | 1.00 | 82.98 |
5': 16S:5
3': 23S:3
Itai Sharon
(11/18/12)
|
|
CARSEP_15
CARSEP_616
Species:
Staphylococcus epidermidis (94.74%)
|
38 | 39101 bp | 32.00 | 1.00 | 82.82 |
5': Scaf_662:3->Scaf_616:2932:5
3': 23S:3
Based on read mapping and comparison to RP62A - remove Scaf_616:1-2932.
Itai Sharon
(11/18/12)
N-segment on (6318, 6839) could be resolved.
Itai Sharon
(11/18/12)
|
|
CARSEP_5
CARSEP_662
Species:
Staphylococcus epidermidis (77.27%)
|
22 | 24591 bp | 29.07 | 1.00 | 86.04 |
5': ~1.8 Kbp (possibly 666)->661:5
3': Scaf_616:2932:5
See comment for Scaf_616:1-2931
Itai Sharon
(11/18/12)
5': CARSEP_666:5
Itai Sharon
(11/18/12)
|
|
CARSEP_11
CARSEP_618
Species:
Staphylococcus aureus (57.14%)
|
21 | 19756 bp | 30.03 | 1.00 | 71.23 |
5': CARSEP_668:3' (transposase)
3': CARSEP_663:5' (AT-rich region on both ends, one insert connects the two)
3' hits a dead-end, but:
1. there is one paired-end read that connects this one with 663:5, and
2. by reconstructing the region after 618:3 it was possible to find 2 other paired-end reads that were connected to 663:5
Itai Sharon
(11/18/12)
Verified the assembly of this genome as well as its origin (CARSEP). Transposase on the 5' end is a Staph epi one. This one is interesting because it has a 100% identity to a Staph aureus piece and it also contains a second Arginine Deiminase Operon.
Itai Sharon
(11/18/12)
|
|
CARSEP_21
CARSEP_648
Species:
Staphylococcus epidermidis (82.35%)
|
17 | 19652 bp | 32.67 | 1.00 | 85.69 |
3': 23S rRNA:3'
Itai Sharon
(11/18/12)
5': CARSEP_668:5
3': CARSEP_668:5
Itai Sharon
(11/18/12)
|
|
CARSEP_19
CARSEP_663
Genus:
Staphylococcus (85.71%)
|
14 | 12008 bp | 30.45 | 1.00 | 75.42 |
5': AT-rich region with low coverage, one insert connects it to CARSEP_618:3' (see comment for 618)
3': Scaf_654:3 (need to remove ~2 Kbp after position 10454)
For the 3' end, Scaf_654 mini-assembly connects it to 663:10454:3, other way around does not work, possibly because this one also connects to the plasmid Scaf_620. 654 and 618 are next to each other on RP62A.
Itai Sharon
(11/18/12)
This may be a plasmid.
Itai Sharon
(11/18/12)
|
|
CARSEP_13
CARSEP_654
Genus:
Staphylococcus (100%)
|
8 | 7851 bp | 30.61 | 1.00 | 80.59 |
5': Scaf_614:3
3': Scaf_663:10454:3
5': see comment for 614:3. Also: region 1-~6400 is present in both St. 1 and 3, 6400-7800 is clearly not present in St. 3. The coverage for this one is low, but pattern is similar to St. 1. Connections are hard to resolve, possibly it exists on only part of St. 1's members
Itai Sharon
(11/18/12)
|
|
CARSEP_16
CARSEP_621
Species:
Staphylococcus epidermidis (80%)
|
5 | 5759 bp | 30.40 | 1.00 | 64.39 |
5': CARSEP_668:3
3': CARSEP_668:3
Itai Sharon
(11/18/12)
|
|
CARSEP_22
CARSEP_657
Species:
Staphylococcus epidermidis (100%)
|
3 | 4224 bp | 32.72 | 1.00 | 66.62 |
5': CARSEP_668:3
3': 23S:3
Itai Sharon
(11/18/12)
|
|
CARSEP_24
CARSEP_666
Species:
Staphylococcus warneri (50%)
|
2 | 2675 bp | 29.20 | 1.00 | 86.24 |
This one may come between CARSEP_661:5 and CARSEP_662:5. It does not connect very well to any scaffold, and is also low-abundance related to the genome (but has the same pattern as St. 1).
It carries a transposase and therefore is probably some type of mobile element. In any case it does not carry anything important.
Itai Sharon
(11/18/12)
|
|
CARSEP_25
CARSEP_668
Species:
Staphylococcus sp. DORA_6_22 (100%)
|
1 | 1120 bp | 32.32 | 1.00 | 90.00 |
5': CARSEP_660:5, CARSEP_660:3, CARSEP_652:5, CARSEP_647:5, CARSEP_606:3, CARSEP_607:3, CARSEP_613:5, CARSEP_648:5, CARSEP_648:3, CARSEP_671:5
3': CARSEP_644:5, CARSEP_644:3, CARSEP_604:5, CARSEP_604:3, CARSEP_607:5, CARSEP_613:3, CARSEP_618:5, CARSEP_621:5, CARSEP_621:3, CARSEP_657:5
Itai Sharon
(11/18/12)
|