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Project: AAC Analysis

Owner: Rose Kantor

Description:

Kantor RS, Wrighton KC, Handley KM, Sharon I, Hug LA, Castelle CJ, Thomas BC, Banfield JF. 2013. Small genomes and sparse metabolisms of sediment-associated bacteria from four candidate phyla. mBio 4(5):e00708-13. doi:10.1128/mBio.00708-13.

The genomes unique to this project are RAAC1, RAAC2, RAAC3, and RAAC4 where RAAC is short for "Rifle Acetate Amendment Columns." Other publicly available genomes from related organisms are included for comparison (Podar 2007, Wrighton 2012, Rinke 2013, Albertsen 2013, and Campbell 2013).

Briefly, the sequencing data used to reconstruct the RAAC1-4 genomes are from 13 sediment columns amended with acetate and one unamended sediment sample. The column amendment experiment was conducted at the Rifle Integrated Field Research Challenge site (Rifle, CO, USA), sponsored by the Department of Energy, in 2010.

Additional data sources

Albertsen, M., Hugenholtz, P., Skarshewski, A., Nielsen, K.L., Tyson, G.W., and Nielsen, P.H. (2013). Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes. Nat. Biotechnol. 31, 533–538.

Campbell, J.H., O'Donoghue, P., Campbell, A.G., Schwientek, P., Sczyrba, A., Woyke, T., Söll, D., and Podar, M. (2013). UGA is an additional glycine codon in uncultured SR1 bacteria from the human microbiota. Proc. Natl. Acad. Sci. U.S.a. 110, 5540–5545.

Podar, M., Abulencia, C.B., Walcher, M., Hutchison, D., Zengler, K., Garcia, J.A., Holland, T., Cotton, D., Hauser, L., and Keller, M. (2007). Targeted access to the genomes of low-abundance organisms in complex microbial communities. Applied and Environmental Microbiology 73, 3205–3214.

Rinke, C., Schwientek, P., Sczyrba, A., Ivanova, N.N., Anderson, I.J., Cheng, J.-F., Darling, A., Malfatti, S., Swan, B.K., Gies, E.A., et al. (2013). Insights into the phylogeny and coding potential of microbial dark matter. Nature.

Wrighton, K.C., Thomas, B.C., Sharon, I., Miller, C.S., Castelle, C.J., VerBerkmoes, N.C., Wilkins, M.J., Hettich, R.L., Lipton, M.S., Williams, K.H., et al. (2012). Fermentation, hydrogen, and sulfur metabolism in multiple uncultivated bacterial phyla. Science 337, 1661–1665.